Reply to two prompts in 100 words each. No sites needed, these are just thoughts on the prompts. ( Prompt 1: Streptococcus pyogenes is a mature biofilm, known to consist of proteins, DNA, and a polysaccharide containing material known as glycocalyx. Throat cultures have been developed to optimize the identification of Streptococcus pyogenes. Performed cultured throat swabs on a 5% blood agar with trypticase soy base incubated in air remains the gold standard and reference method for the diagnosis of Streptococcus pyogenes acute pharyngitis (rose, 2020). The PYR test is a rapid colorimetric method often used to distinguish Streptococcus pyogenes from other β-hemolytic streptococci with a similar morphology and tests for the presence of the enzyme pyrrolidonyl aminopeptidase. This enzyme hydrolyzes L-pyrrolidonyl-β-naphthylamide (PYR) to β-naphthylamide, which produces a red color when a cinnamaldehyde reagent is added. The test can be performed on paper strips that contain dried chromogenic substrates for the pyrrolidonyl aminopeptidase within a few minutes (Young, 2016). The direct identification of Streptococcus pyogenes from blood culture bottles enables the determination of a suitable antibiotic treatment. The majority of Streptococcus pyogenes isolates encountered in the clinical laboratory, serologic tests, in conjunction with presumptive physiologic tests offer an acceptable and cost-effective alternative available identification systems. Prompt 2:
