Create one powerpoint slide for the poster. The poster should contain at least t
Create one powerpoint slide for the poster. The poster should contain at least three figures: one (1) highlighting the secondary structure and two (2) active site images. Each Figure must have a captionfor the active site images, be sure to describe the types of interactions you are showing in the image, and refer to any specific amino acid residues (involved in catalysis or inhibitor binding) by their amino acid number. The PDB is 2Z5X. I included an example of how the poster should look like for the PyMOL image part in the file. Full instruction: A. Go to the RCSB PDB and examine all ligands bound to the protein structure. One of these will be your drug, but there may be others. List all of the ligands and their role (e.g., substrate, product, coenzyme, inhibitor, cofactor, ions unrelated to function etc.) Note that the ligands are listed on the structures PDB entry page. Insert at least one PyMOL image zoomed out to show your entire enzyme. Be sure that each of these molecules is clearly visible in your snapshot; you might label these in PyMOL or in the program you create your poster in. Some ligands are not relevant to your enzyme’s biological function (i.e., are only there due to crystallization conditions and/or are not located at a site in the enzyme where they give information about the true substrates, products, cofactors, or regulators). You will need to read the manuscript and potentially the experimental procedures to figure out a molecules role in the structure. B. Create at least two PyMOL snapshots zoomed in on just the active site of your enzyme. List the catalytic residues (e.g., Ser261, His342). Clearly show these residues in your snapshots. Highlight other molecules in the structure that are involved in the reaction catalyzed by your enzyme (substrates, products, cofactors, coenzymes, etc.). You may hide the bound drug/inhibitor to create this image. C. Zoom in on the drug bound to your enzyme (note that it may be in the active site, or bound to another part of the protein, depending on the type of inhibitor). Name the molecule that you have chosen. Illustrate all noncovalent interactions between this molecule and the enzyme using at least one PyMOL snapshot. Include hydrogen bonds, hydrophobic packing, and salt bridges. Describe all of the polar interactions, referencing the specific amino acid side chain that is involved in the interaction by its three-letter code and residue number and describing how it contacts the drug. Also highlight one nonpolar interaction. have attached exmples
